Nuclear Magnetic Resonance

The laboratory of CERMANU contains two magnets Bruker Avance Ultrashield da 300 e 400 MHz.

 The probes used in the 400 MHz magnet are the following:

- BBO 5mm direct probe for the multinuclear analyses in liquid state; all 1D and 2D NMR techniques are conducted.

- BBI 5mm direct probe for the multinuclear analyses in liquid state (inverse T/R coils to improve resonance of ¹H nucleous in respect to X nuclei; all 1D and 2D NMR techniques are conducted.

- uTXI 0.8-1.6 mm microprobe, for ¹H, ¹³C and ¹⁵N analyses in liquid state; small samples volumes can be loaded in  100 μL NMR tubes; dedicated to trace samples but highly soluble in deuterated solvents; all 1D and 2D NMR techniques are conducted.

- HRMAS with 4mm rotors (15kHz max rotation speed) for ¹H e ^{13C analyses of semi-solid samples (fresh tissues, gels, whole food samples, etc.); all 1D and 2D NMR techniques are conducted.}

The probes used in the 300 MHz magnet are the following:

- CPMAS with 4mm rotors (15kHz max rotation speed) for multinuclear analyses of solid state samples;

- High Power Diffusion (HPD) probe for 5 e 10 mm tubes, for protons diffusion experiments (Diff30) at high power on highly viscous samples (biological and mineral oils, waxes, thick colloids, etc.);

- ¹H-MRI (Magnetic Resonance Imaging) for spectroscopic analyses in 5, 8 and 10 mm tubes; proton-based magnetic resonances images are obtained through measurements of Spin density, T1, T2 and Diffusion.

- ³¹P-MRI (Magnetic Resonance Imaging) for spectroscopic analyses in 8 mm tube for the ³¹P nucleous.

Mass Spectrometry

GC/MS. Gas Cromatography/Mass Spectrometry. One Perkin Elmer TurboGold GC/MS with long quadrupole for analyses of compound up to 200 Da, by both electronic impact and chemical ionization.

LC/MS. Liquid Cromatography/Mass Spectrometry. A Shimadzu systems couple an ultra-high performance liquid chromatography (UHPLC) to an Electrospray (ESI) ionization source to a mass spectrometer consisting in an ion trap and a time of flight (IT-TOF). This LC/MS system detects molecular ions with an accuract up to the fourth significant digit, and is capable of MS¹⁰ fragmentations of ions before detection.